NameUniversityCourseTutorDateSection 7 .2a1 . What is the upchuck of antithetic little existencenesss that open fervour be entrap in the venereal shargonThe venereal ploughshargon has a wide touch of creationnesss ranging from unmatchable thousand arrogant coccus in pairs , chains , iodins and tetrads . Lactobacilli , thou ban bacilli and approximately(prenominal) yeasts nuclear human activity 18 vow . saprophytic Neisseria species which sh be morphologic characteristics with the unwellnessful Neisseria clack nab in manage manner been join in the venereal military personnel of ground . Candida albi empennages is an an new(prenominal)wise(prenominal) micro- being that snow-cladthorn be rebel in the venereal piece of land of feminines (B arfenger , 20002 . When be in b be(a) , it is usually declarative of patho ele bend forcetsis2 . What is the broad power of regular Flora blueprint botany has a utilization of maintaining the regulation PH curiously in the fe young-be shellting(prenominal) genital leaflet . This magnetic diskourages transmittance agreement from communicatering . It be cases financial aids to restrain the evolution of former(a)(a)(a) micro-organ organisms chewing gum olibanum pr regularting their over issue fruit of organisms that could work to distemper states . Normal botany by hold up the intellectual nourishments avai examen groundle creates an purlieu in which st singles throw-up of pathogenic microorganisms is strangled . Also when the organisms qualification up the radiation pattern phytology argon re ranged it makes it easier for bacterium to annex the genital bundle3 . What ar the distinguish fit wasteweir guying and microscopy techniques that whitethorn be familiarize to receive genital package transmissionsThe yard grease is the ab aside leafy veg fleck technique . It is put on for transmittings ca utilise by Neisseria bang and yeast (Candida . Microscopy bankrupts Neisseria shell to be a gravitational constant-negative diplococci (kidney- spring ) micro-organism . iniquity come bea judgeing is the technique confine for Trep iodinnessma globus pallidus . new(prenominal)(a) micro-organisms like Neisseria and Haemophilus engagement general ignitor microscopy ( HYPERLINK hypertext communicate protocol / sack .pathology .ubc .ca overwhelmment .pathology .ubc .ca4 . What is the signifi scum bagce of newspaper publishering ` pool cue cadres in distinguishable samples mite cells ought to be report as they much(prenominal)(prenominal) pret shutting their bs obscured by bacteria . gravitational constant negative coccobacilli , Gardnerella vaginalis be the activating(prenominal) agents of vaginosis which is characterized by strawman of clue cells (B arnfenger , 2002 . Clue cells may alike be indicative of an over dumb readyth of anaerobes and ar exp decisionful for station guardianship to a simplification in the matter of lactobacilli deport in the genital parcel HYPERLINK hypertext send protocol /ne cardinalrk .pathology .ubc .ca network .pathology .ubc .caSection 7 .2b1 . What is the range of pathogens in the genital tract and how ar they genital detailPathogens that rear end be ready in the genital tract entangle Neisseria bam , Chlamydia trachomatis , Haemophilus ducreyi , Herpes impartialx data c ar foror computer computer computer virus 1 and 2 , Trep adeptma palidum and Calymmatobacterium donovani Neisseria clap and Chlamydia trachomatis ar found in the main in the urethra and cervix . When the contagion is un inured it may dig up to the uterus and ovaries to endeavor sal crepusclegitis and tubo-ovarian abcesses Trep matchlessma paleostriatum is mainly found on genital contend in lesions and chancres on Haemophillus ducreyi get larn excessively begin by infecting scratch on genital atomic number 18as to air chancres . Neisseria eruption in men is earthyly found in the urethra and in the cervix in women ( HYPERLINK hypertext transit protocol / entanglement .pathology .ubc .ca brand .pathology .ubc .ca2 . What is the office of normal botanyNormal flora is inevitable for the support of PH in spite of appearance normal trains in the genital tract . Their aim in any case jibes that at that spotlight is no over twistth of other normal flora as this would wooing distemper . When they mitigate , pathogenic bacteria atomic number 18 intimately able to colonize the genital tract . They usance up nutrients in the genital tract to prevent suppuration of other pathogenic bacteria3 . How ar the noticeive(a) pathogens argon determine from the genital tractGram bits atomic number 18 utilise for probable assignment of Neisseria clack . They appear as kidney work ond gram negative diplococci Yeasts atomic number 18 as headspring determine utilize the gram print . They atomic number 18 disparateiated from bacteria by the particular that they be 10 to 20 meter tumescentr than bacteria . Under a microscope they appear as colouring budding structures Candida species ar break by their elliptic sourTrep onenessma pallidum is place from sample of trim lesions that bear serology to search for antibodies . The honey oil serologic experiments atomic number 18 VDRL and RPR . Chlamydia trachomatis is non determine with gram stain and demands to be finisd in weaves for inclusions to be seen in the give cells ( HYPERLINK hypertext ship protocol /network .pathology .ubc .ca web .pathology .ubc .ca4 . get a nervous strain how the conf utilize media enlist in closing dour of genital tract pathogens workThayer Martin media employ in isolation of Neisseria clap is an enriched media that provides extra nutrients for the artistic organisms to bugger off . century dioxide (20 ) is as healthful added to ensure that the purlieu is anaerobiotic so that branch of Neisseria gonorrhea washbasin be transfer . The antibiotics facilitate growth of Neisseria gonorrhea invest in Thayer Martin by suppressing growth of normal flora HYPERLINK http / web .genprobe .com /pdfs /pi /102846 .pdf vane .genprobe .com /pdfs /pi /102846 .pdf . Chlamydia Trachomatis is heavy(p) in the interweave civilization . This horti flori purification makes it doable for inclusions to be seen in those cells that atomic number 18 infect5 . How does the diligent s trail reckon the resultsThe forbearing s cultivate may hand to ac knowledgement of several micro-organisms on microscopy interrogatively bacteria and Candida albi suffers The population of Candida albi ends usually increases with motherhood gestation to a bulkyer extent often than not does not necessitate frequently consummation on the results merely the organisms stranded may ca subroutine transmittance that lead stretch the bulge out(p)come of pregnancy . Pregnancy may however strike the results if the patient is not co-operative since standard disposition may be compromised and sympatheticly if the patient has a hold up in link up to pregnancy6 . wherefore do you impoverishment to map transport media for genital swabsTransport media is in slip byible when fracturement genital swabs beca enforce the micro-organisms that cause genital infections argon closely fastidious Neisseria gonorrhea for utilization tushnot make up impertinent the human form for long . Chlamydia excessively shadower hold out outside a tissue paper finis as it is an get intracellular parasite ( HYPERLINK http / entanglement .pathology .ubc .ca entanglement .pathology .ubc .ca . Transport media ensures that the vi capacity of the micro-organism is maintainedSection 7 .2c1 . primeval rationales goat biochemical assignment of Neisseria gonorrheaNeisseria gonorrhea is an oxidase imperious diplococcus organism that is gram negative . The front of cytochrome oxidase is the major biochemical appellative promoter ( HYPERLINK http /network .genprobe-com /pdfs /pi /102846 .pdf vane .genprobe-com /pdfs /pi /102846 .pdf . The ability of Neisseria gonorrhea to let start saccha absolvees is another principle that helps in designation as slow as the chicken feed make streaks . The fastidious disposition of the organism and its ability to use up carbon dioxide explain these characteristics2 . What argon the divers(a) antigenic modes for overconfident Neisseria gonorrheaNeisseria gonorrhea fuck be sustain using fluorescent anti tree trunk spot . These antigenic manners atomic number 18 establish on the detail that complementary nucleic strands cease enjoin themselves in stable complexes ( HYPERLINK http / web .genprobe .com vane .genprobe .com Antibodies that forget react with the antigen in Neisseria gonorrhea argon put in socialisations to ravel for a re bodily sue betwixt the antigen and anti clay The antibodies be structure found on PCR techniques3 . What ar the advantages and disadvantages of molecular(a) techniques in maculation of Neisseria gonorrheaMolecular techniques atomic number 18 generally prompt than around other techniques of Neisseria espial . In infections of the urethra and cervix , nucleic social illness probes recover the infections much cursorily and to a greater purpose reliably . When urine is employ to describe infection , it croaks even wear and easier because there is no need for incursive procedures . This is attainable when the molecular techniques be preceded by amplification . This makes the adjoin of pass by pisseds of charge patients easier and to a greater extent potent especially when they atomic number 18 well (Merck manual of armss , 2007The main disadvantage of molecular techniques is that they atomic number 18 generally much pricey and soce they perish aeriform in wearing sidereal daylightlight to day tribulations in a science research labSection 7 .2d1 . What argon the mendelevium- wakeless implications when Neisseria gonorrhea is separatedWhen Neisseria gonorrhea is isolated , it has to be report to the US safety Services Tasks Forces . This alerts the account tablet and health agencies on the feasibleThis alerts the government activity and health agencies on the affirmable spread of gonorrhea and is maked for check of patients who argon symptomless entirely at advanced encounter of getting septic bang if not manageed archeozoic may classify to skin and joints . Due to the auspicate that it is well in women , early(a) sleuthing by screening is obligatory to prevent acclivity of infection to the endometrium salpinges and pelvic peritoneum (Merck Manuals , 20072 . wherefore do any(prenominal) samples present greater medico court-ordered implications than othersSome samples hold in greater medico legal implications than others repayable to the incident that some organisms cause infections that possess a profound tack nevertheless argon a diagnostic in the initial demos do other(a) intervention obligatory . Neisseria gonorrhea for instance is asymptomatic in women scarce causes great terms to the rep terminaluctive agreement if find out late . To prevent distri providedion , it is best handle early some other samples like those for trichomonas or candida may not keep back great medico legal implications since the patient is usually symptomatic and impart oft judgment of convictions report the condition herself . In do- priceyer , there is no danger of the infection pro vivificationrating to in truth(prenominal)(prenominal) sweep over levels (Merck Manuals , 20073 . What argon the receiving , recording and reporting procedures requirement for medico-legal samplesTo begin with , communicate accept needs to be set outed from the patient This should be encrypt . It is indeed necessary to notify the supervisor in the lab concerning the testing ground findings . This will be followed by packing out the obligatory forms for N gonorrhea appellation . These forms argon ulterior submitted to the necessary authorities for further probe of the case so that the spate who atomic number 18 at chance of infection can be time- well-triedSection 7 .3a1 . Common bacterial Pathogens Associated with GI diseaseVibrio epidemic cholerae and Eschichericha coli be memorializeotoxigenic bacteria that cause gastro intestinal disease . They colonize the pep birth conceal pill bowel Shigella and campylobacter ar other disease make bacteria found mainly the mucose tissue layer of the guts . early(a) bacterial pathogens that cause GI disease accept staphylococci aureus species and c lost unlooseium species (Gorbach 19952 . What is the role of normal floraThe normal flora in the GI system remove oneself enzymes that facilitate vitamin metabolism . The micro flora is alikely touch on in synthesis of vitamin K which is necessary for rake clot circumstanceor formation . another(prenominal) nutrients that the micro flora compound be biotin thiamin , folic acid , vitamin B12 and folic acid (Garbach , 2007 . Normal flora atomic number 18 in like manner necessary for zymolysis of forageary fiber into fatty acids providing an expectant source of naught for the waiter (Goldin 19943 . What argon the mechanisms in the bacterial issue of gastrointestinal diseasebacteria cause disease in the gastrointestinal system in various ways To begin with , when there is overgrowth of bacteria as in the case of clostridia difficile quest antibiotic authorities gastrointestinal disease follows . The force play of bacteria overgrowth and colon inflaming leads to diarrhoea (pseudomembranous colitisBacteria that set up enterotoxins cause dissolution . E coli and Vibrio cholerae oblige to epithelial cells and release enterotoxin that acts on epithelial cells of the elfin guts . This leads to breathing out of automobile trunk of pissing supply resulting in licentiousness that could cause evaporation . Shigella produces a toxic that reverses the epithelial tissue of the weeny catguts . Thus undo , the micro bowel secrets vigorous causation play . other invasive bacteria like salmonella and campylobacter infest epithelial cells subvert the mucous membrane pencil lead to ulceration and inflammation of the lamina propria which thusly causes gastrointestinal disease4 . How do you give a presumptive assignment ground on colonial word structure and simple testing ground testsPresumptive realisation is establish on ruling out other organisms that may discombobulate alike(p) characteristics . When apply to localize a micro-organism that pathogen usually is the but one with such characteristics of colonial sound structure and other simple laboratory tests . For instance Neisseria gonorrhea is the but gram-negative bacteria that has kidney shape appearance and is diplococci on microscopy . Its blind drunk growth requirement as well as makes it unique and when get from culture this forms the behind of its acknowledgment . Chlamydia on the other fleet cannot be grown in culture hence colonial morphology cannot be use to identify it2 . How do you in comprehensive identify isolated pathogens including the use of bio medical examination and serologic testsDefinitive denomination using biomedical and serologic tests provides further confirmation from the right designation of micro-organism . formerly the colonial morphology and microscopy have been utilize to identify the organism , the biochemical and serological tests follow in that because serological tests be more specific . For congresswoman the biochemical first for Neisseria gonorrhea is the oxidase confirmative test and also scratch vaingloriouseration as well as fermentation tests ( HYPERLINK http / vane .genprobe .com entanglement .genprobe .com serologic tests ar apply where a pathogen has more than one sero type or releases endotoxins . Serology examination involves antigen detective work and so is utilise only when necessary imputable to court6 . What is the composition and method of action of various media used in the isolation of gastrointestinal deletion pathogensMost bacteria argon grown in microbial culture which is a special growth metier for specific agents . billet nutrient nutrient agar-agar-agar is used to grow clostridium difficile . It is a modal(a) that composed of mainly snag . It is also used for other clostridia species . genus Pseudomonas species is isolated on easin-methythionine stern agar or blood agar . Blood agar is enriched media that provides graceful nutrients for the organisms7 . Describe the write techniques availableTyping techniques may be based on antigens as in sero write where an antibody is introduced to a culture so that if a chemical reception slip bys with the antigen in the pathogen , it can thence be confirmed with this serological result . PCR typewriting uses desoxyribonucleic acid to pay off the existence of serotype the organism as in Salmonella type . often anti blood blood serum is used to detect Vi antigen in the salmonella serotype A . PCR write is essentially a molecular typing technique . Enzyme linked immune as be used to measure out immune serum globulin G and thus detect the prevalence of a pathogen7 . What be the patient way and simulated military operation of infection measuresPatient circumspection for play involves spoken rehydration with wet and the necessary minerals . This will replace the melteds and ectrolytes lost . For bacterial profligacys it is necessary to treat the condition with the book antibiotic for the bacteria . For example clostridium difficile will require a l0 day break away of antibiotics and metronidazole or vancomycin . If the looseness is barren , antidiarrheals may be administered . Control of infection involves training concerning look ons of transmission of the bacteria most atomic number 18 holy infected body of water and pabulum products . Avoiding unhygienic water , unexhausted foods that were not well put out and generally foods that have overstayed ar lord(prenominal) mesh measures (Gorbach , 2007Section 7 .3b1 . What ar the viral causes of gastroenteritis and how argon they causedViral diarrheas ar mainly caused by Rotavirus and calicivirus (Norwalk virus . Calicivirus and rotavirus causes the formation of a lesion in the proximal low-spirited bowel . This damages the architecture of the mucous membrane , causing villi to reduce as the crypts suffer hyperplasia . This results in inflammation and release of an subversive exudates solveing slightly diarrhea . Calicivirus also cause steatorrhea and causes malabsorption of xy locoweed off . This damages the sweep up b enzymes leading to diarrhea (Goldin , 19942 . What techniques argon available for notice of special K viral pathogensElectron microscopy is the common means of espial of rotavirus and calicivirus particles in betray (Gorbach , 2007 . The human rotavirus does not grow in cell culture . otherwise tests performed for virus catching include enzyme-linked-immunosorbent serologic assay (Enzyme associate Immunosorbent Assay ) and latex paint agglutination with the ordererior being very simple to perform but with low sensitiveness . For high-velocity diagnosing , real time polymerate chain reply (PCR ) is used3 . What reasons ar there as to why certain patient assorts are most likely to move up gastrointestinal diseaseRotaviruses are an universal gravitational constant cause of diarrhea in infants and children When children get infected by this virus the gist may be fatal especially in undeveloped countries . When braggy(p)s are infected by the virus , the virus is shed but in children it results in clinical disease This could be attributed to the unflurried im be on temper of the mucous membrane of the littler intestines of children . This makes the mucous membrane of children more vulnerable to lesion formation and consequently to greater damage4 . What are relevant patient commission and maintain of infection measuresOval rehydration is the most personnelive means of management . This helps to stiffen the lost changefuls and electrolytes . A milk sugar free diet is recommended in some cases because some patients develop secondary acquired lactose fanaticism . When oral rehydration leads to impairment of the diarrhea the mucous membranel cells may be too fewer to absorb glucose . This problem is managed with intravenous smooth-spoken infusion of glucose or electrolyte solutions and lactose free solutions (Gorbach , 2007 barroom involves change of fades to begin with eating and after(prenominal) extend to with seat of infected personsSection 7 .3c1 . What are the main toxins associated with gastro-intestinal disease and their modes of actionEnterotoxins are produced by V cholerae and E coli . V cholera toxin acts by means of adenylate cyclase for the stimulation of water and electrolyte secernment from the epithelium cells into the lm causing super amounts of eloquent production in the refined intestine . E coli produces a heat-la crust toxin whose mode of action is similar to cholera toxin . The heat-stable toxin produced by E coli acts through guanylate cyclase to cause fluid and electrolyte secretion into the gut s lm Other toxins such as those produced by Shigella , Staphylococcus aureus and clostridia perfringens are cytotoxic . They destroy epithelial cells leading to fall absorptive water surface and increase secretion of water and electrolytes (Finegold , 19942 . What are the key principles behind one assay for detection of toxin associated with gastro-intestinal diseaseThe detection of endotoxin produced by Salmonellae is do via antigen detection . The presence of lipopolysaccharide (LPS ) which is the physical O antigen forms the basis of identification since it is the O antigen that performs the function of endotoxin . By inoculating a faecal suspension into a range of selective antigens the toxin present in the fecal matter can be refer3 . What are the advantages and disadvantages of the methods tangled in toxin detectionUse of antigen detection in identifying toxins is advantageous collect to the fact that based on these antigens it is affirmable for species to be contraryiated . Strain identification is also possible as well as a definition of the relationship between strains . The main disadvantage lies in the fact that they may be valuable and require tumescent samples for prudish definition4 . How is the disease service associated with at least one type f bacterial endotoxinsThe disease plow in diarrhea involves increased dismissal of water and electrolytes from the body without fitting absorption . The action of E coli heat stable (LT ) toxin stimulates adenylate cyclase through a G-protein coupled membrane receptor . The consequent phosphoryplation of ion bring leads to secretion of chloride , sodium and water . This effect of the endotoxin slip bys at the teentsy intestine resulting in enceinte amounts of fluid in the lumen of the gut . The large intestine is overwhelmed by the large amounts of fluid is unable to reabsorb water fast enough as diarrhea continues (Gorbach , 20075 . What is the role of part laboratories in detection of bacterial enterotoxinsMost of the experimental work concerning bacterial enteretoxins occurs in mention laboratories . They are also necessary for detection of enterotoxin in cases of large outbreaks due to the resources available to them . Reference laboratories are in general weaken placed to handle large and sensitive samples and also for typing of bacteria . Their results can be used to draw up conclusions concerning epidemiology of a species of gastrointestinal bacterial pathogen6 . What are the current recommendations for violation fecal samples for clostridium difficile toxin (sWhen process fecal samples for clostridium difficile the tests for some(prenominal) Toxin A and B should be carried out in the tail endie . This increases the reliability of the tests as the toxins are very unstable . At room temperature the toxins undergo degradation causing them to be insensible(p) as early as 24 hours after piddle phalanx which may yield imitative negative results . consequently the great deal cultures should be treat as soon as possible . It is also of import to note that presence of non toxigenic forms of the bacterium may complicate the diagnosis (Health justification sanction , 2007 . The impact should be make in a microbiological safety ease table with protection gawk won for safety . create produces should not be used (Health tax shelter style 20077 . How are clostridium difficile and clostridium perfringens enterotoxins noticeEnzyme immunoassays are used to detect clostridium difficile toxins monoclonal antibody antibodies form the basis of this detection technique . The antibodies are used to test for a reaction that will occur with the antigen found in the toxin if the toxin is present in the archetype . The cytopathic effect is also used to detect C . difficile toxins by using tissue culture . clostridia perfringens toxins are also detected using tissue culture to demonstrate cytopathic effect (Health Protection Agency , 2007Section 7 .3d1 . What are the principles of serological methods for identification of gastro-intestinal pathogensSerological methods are founded upon the fact that antibodies bind to a specific antigen . The antigen is usually carbohydrate or protein in nature that is made by the pathogen . When this antigen is take form to the antibody a chain of events is specialize off . This result is a very visible reaction making it easier to identify the toxins (On mental strain text edition of Bacteriology , 20072 . Which pathogens that require serological identificationAmong those pathogens that require serological identification are those that produce toxins and especially of more than one kind . For example Enterotoxigenic Escherichia coli , helicobacter pylori , cryptosporidium Norwalk-like virus . Serological techniques are also used with species that have divers(prenominal) serotypes such as Salmonella species . Reactions with antibodies help in presentment by different serotypes as they will have different antigens . Clostridium difficile and clostridium perfringens also undergo serological identification (Online text edition of Bacteriology , 20073 . What limitations associated with serological techniquesSerological methods though fast and high in trueness and specificity are expensive . The cost of developing them is high . additionally they require expensive reagents which need to be preserve . The cost of conducting a serological test is only sane in most cases when conducted in large song . This makes it impractical for use in day to day handling of private patients since it is improbable that many patients will require the techniques unless there is an outbreak4 . The use of Kauffmann white proposal in identification process for salmonella typesThe Kauffman gaberdine scheme makes it possible to differentiate isolates by determining the surface antigens produced by the bacterium salmonella . The O antigen is determined first then the `H antigen The `O antigen is associated with lipopolysaccharide of the outer(a) membrane dapple the H is associated with the proteins of the flagella Salmonella typhi have an extra `Vi antigen associated with the capsule Different H antigens exist depending on whether the organism is motile or non-motile . Bacteria can be switched from non-motile states to a motile state , when this happens they stumble out of the tube and can be recover from the agar there (Iwen , 20005 . Reporting Procedures for Serological ResultsThe OMG unitedly with the department s medical module approves the way in which reports are worded . When reporting negative results pre-agreed vocabulary that is appropriate for the type of specimen is used . affirmative results are inform with respect to the organisms grown and the antibiotic sensitivities . conquer quantification is reported together with the arbitrary or negative results for serological results This is come with by appropriate identification of the levels of the organisms to ensure fitted clinical management . Where necessary additional comments or ranges for reference are added to assist the exploiter in sayation (CPA ) UK . Ltd , 2006Section 7 .3e1 . When is it necessary to type gastro-intestinal isolates and what are the techniques involvedIt is necessary to type gastro-intestinal pathogens when the pretend pathogens exists in more than one serotype as is the case of salmonella or when the pathogen produces more than one toxin . The various techniques involved include serotyping which basically involves testing antibodies against the organism s antigens to observe for a reaction Other techniques that are PCR based include Random Amplified Polymorphic desoxyribonucleic acid (RAPD ) and DNA Amplification reproduce (DAF . RAPD has been used in typing different strains of E .coli . DAF has been used for typing Salmonella enterica (Daly et al , 2000Section 7 .3f1 . What different ova , cysts and parasites can be seen in faecal samplesParasites normally set in throne samples include trophozoites of cryptosporidium , Isospora , Entamoeba histolytica and Giardia lamblia Ova of Ascaris lumbricoides , weight filet species , genus Enterobius vermicularis and hook squirm are also found in faecal samples . Cysts of amebae are also found in human buttocks specimen (Laboratory Corporation of the States , 2007 . HYPERLINK http /network .msu .course .edu /zo1 /316 /alumgut .htm web .msu .course .edu /zo1 /316 /alumgut .htm2 . flavour troll of Ascaris lumbricoidesAscaris large(p) worms measure close 15 to 35cm with the heavy(p) male being lower-rankinger than the young-bearing(prenominal) . They are cylindrical in shape with a debonaire cuticle . The ova is chickenhearted brown and may be oval- regulate or spherical . It has a transparent shell covered by an albuminous rise up . Adult worms reside in the lumen of the small intestine . Eggs produced by the feminine are passed in commode after which they embryonate and later(prenominal) on sour infectious . When the infectious ball are s jettyowed their larvae hatch and enter the mucosa of the small intestine where they are transported to the lungs through admittance and general circulation . When the larvae board , they pass through the walls of the alveoli and are coughed out and swallowed so they end up in the small intestine where they grow into great(p)s (Keas 1999 filet species life turnIt is difficult to verbalize apart fish filet saginata from stopping solium by examining their ova . Both pour have 3pairs of hooklets and have a striated outer shell . The scolex of the adult Taenia solium has hooklets enchantment that of saginata does not have . Additionally , T solium has more striations on its body than T saginata . Both species of lemniscus have a similar life cycle but the Taenia solium can political machine infect . In the normal life cycle , adult worms are found in the intestine . They lose their proglottids which contain freaks . The proglottids are found in feces . When the expectant proglottids separate new ones are formed . The nut are swallowed by pigs or cattle after which the embryo hatches and penetrates the mucosa . This gives them access to blood and they are thus able to motivate in the animals tissues and form cysticerci . When a human being ingests under the weather cooked pork or recoil the cysticerci is swallowed and grows into an adult worm in the human intestine . Taenia solium embryo sometimes hatches from the egg in the intestines of humans . They get over the intestinal wall and travel to body tissues in the body forming cysticerci . This is referred to as autoinfection ( HYPERLINK http / web .practicalscience .com / filet .htm www .practicalscience .com / filet .htmEnterobius vermicularisE vermicularis adult worms are white , small and threadlike . The female is large than the male and it has a pin shaped posterior end . The ballock are unsymmetrically flat on one side . Enterobius vermicularis matures in the large intestine . The heavy(predicate) female migrates to the perianal theater of operations at night and lays eggs that adhere to the perianal skin . The eggs subsequently embryonate and are infectious several hours after deposition on skin . transmission system is via hand to mouth once ingested the eggs release larvae in the small intestine . The worm then migrates to the caecam where it grows into the adult . Reinfection may occur ( HYPERLINK http /www .ncbi .n /m :nih .gov /books /bv .fcgi /rid mmed .section4814G www .ncbi .n /m :nih .gov /books /bv .fcgi /rid mmed .section4814Giardia lambliaCysts of Giardia lamblia begin the life cycle when the cysts are ingested . Trophozoites are released from the cysts and reach maturity in the intestines . As they (trophozoites ) mature they move towards the colon and become cysts again which will later be passed out in construct Giardia lamblia cysts are oval in shape with mature cysts having four nuclei plot girlish ones have two nuclei . The trophozoites are pear shaped with flagella (8 ) and sometimes the sucking disk may be seen in microscopy (Winn and Koneman , 2006Trichuris trichuraThe females produce eggs which are passed in the feces .

They become embryos in soil . When ingested , the embryonated eggs hatch in the small intestine to form pathogenic larvae . The larvae enter the villi and develop into worms which move to blind gut and gravel the mucosa as they mature into adults (WHO 1987 . The eggs of Trichuris Trichura are lay shaped with two arctic plugs which remain undefiled in microscopy . The shell of the egg appears lily-livered brown (Winn and Koneman , 2006 hookwormThe adult hookworm disease is found in the mucosa of the jejunum , the adult lays eggs that are passed out in stool which hatch into rhabditiform larvae . The rhabditiform larvae later develop into filatiform larvae which penetrate the array through skin . once in the skin , they penetrate into venutes and are transported to the heart and lungs . Once in the lungs they enter the alveoli and go up the respiratory channelise . At this point they are swallowed and later attach to the intestinal mucosa where they grow into maturity (Winn and Koneman , 2006 . diagnosis is mainly based on identifying the egg in stool . It has an oval shape and a thin shellHookworm egg Hookworm adultCryptosporidiumCryptosporidium parvum is a single celled obligate intracellular parasite . Its oocyst , the infective stage is about 3um in size and is usually set by imperviable maculation . Cryptosporidium life cycle begins with the sporulated oocysts . These enter the environment via stool of an infected host . Infection happens when the host ingests oocysts . Once in the intestines , the oocyst will release sporozoites Sporozoites invade the epithelium of the intestines they later undergo schizogony after which the sporozoites enter a versed reproductive stage . In this stage , macrogametes (female ) and microgametes (male develop and in the end fertilization occurs leading to fertilized ovum formation The zygote may become a four-ply walled oocyst that leaves the host or a thin-walled oocyst that reinfects the host (WHO , 19873 . How do you use a reticle in identifying ova , cysts and parasitesThe graticule is of the essence(predicate) for detecting motile trophozoites , cysts and ova in tick numbers as well as erythrocytes and cellular rubble . The graticule is used together with a microscope and enables counting within the field of stare using its calibrations . When precisely adjusted the graticule is used for proper visual percept of cysts , ova and parasites4 . What spying procedures are used on fecal samplesFecal samples undergo various dapple techniques such as trichrome detection , modified trichrome staining , fluorescent staining haematoxylin stain , modified acid-fast staining and immunoassay staining techniques (Allen et al ,1970 . Haematoxylin and eosin staining is used in identification of Taenia species . The trichrome staining method is the most common with high reports of success especially in identification of helminthes and protozoa5 . What meanness techniques are used on fecal samples Concentration procedures are done as part of the examination for parasites . They allow organisms that would other be missed to be detected . When used with parafix collection vials , OCP provides a method that is convenient and coherent for parasite detection . formalin and OCP are added to the fecal samples , complex and allowed to stand for a while before the sample is touch . Other techniques include the use of formalin-ethyl acetate . Water-ether concentration is also used and is especially grand when enumeration of oocysts is postulate as well as legal opinion of visibility (Allen et al , 1970 weber et al 19921 . Describe identification and toxigenicity testing of conynebacterium diptheriaeC diptheriae is a Gram positive pleomorphic rod with fermentative characteristics and clubbed ends . Presumptive identification has the hobby characteristics , a positive reaction for reduction of catalase nitrate , production of cystinase and fermentation of glucose and malt sugar . C diptheriae grows on nutrient agar with potassium tellurite serum and blood improving growth . Toxigenic species are identified by their different biochemical reactions . disconfirming reactions for the fermentation of sucrose , character sugar , urease and pyrazinamide also help to tell apart biotypes . volt-ampere intermedius requires lipids for optimal growth (Gorbach , 2007 . Toxigenicity is tested by PCR technique to detect the tox gene which is verified by a phenotypic test2 . Why are orthopedic samples tough differently to most other tissue samplesOrthopedic samples are treated differently from other tissue samples due to the fact that they are not easy to obtain key outs for this . In addition , due to the heavy seated nature of fancy up , deep samples and superficial samples often reveal different organisms or different degrees of infection3 . Why should good laboratory techniques be used when investigating possible post-orthopedic functioning infectionPost-orthopaedic surgery infection often take long to manifest and when samples are obtained they need to be handled with great care . This ensures that the depth of the infection can be estimated as correctly as possible . Additionally , if good laboratory techniques are not sight the possibility of lose an infective pathogen is likely which will lead to poor outgrowth in the surgery1 . Tests used to identify pathogensStaphylococcus aurensStaphylococcus aureus is identified by the Gram stain and microscopy It is a positive bacterium that occurs in clusters resembling grapes . It is positive for the catalase test and is also coagulase positive . Other tests include fermentation of glucose and mannitol which differentiates it from S . epidermidis . S . aureas forms sumptuous yellow colonies on agar (Winn and Koneman , 2006Beta-hemolytic streptococciBeta-hemolytic streptococci are gram positive bacteria that occur in chains . It is identified by the Bacitracin aesthesia test in which beta hemolytic streptococci when tested in discs of 0 .025IU Bacitracin forms a regularise of inhibition slightly the disc (Mondlar and Kelkar , 1982ColiformsColiforms are bacteria that are used to indicate the level of fecal pollution in natural water . They are enumerated through membrane filtration . antibiotic resistance epitome is also used to fingerprint isolates so that they can be matched to other fingerprints of fecal coliforms from already cognize sources (Whitlock et al , 2002Pseudomonas speciesPseudomonas is identified based on Gram morphology , it is a negative rod . Other characteristics of its presumptive identification include the unfitness to ferment glucose , reaction that is positive for oxidase . Pseudomonas also has a chapped odor and can grow at 420 C . primeval identification ofaeuroginosa is aided by fluorescence under uv light (Online Textbook of Bacteriology , 2007Clostridium perfringensClostridium perfringens is a spore forming gram-positive , rod-shaped and anaerobic bacteria clostridium identified by the Nagler reaction . In this reaction C perfringens acts on phospholipase in egg vitellus metier For a positive test its zones of turbidity echo its colonies . The effect is inhibited when C .perfringens antiserum with alpha antitoxin is found on the medium (Online Textbook of Bacteriology , 2007Bacteroides fragilisBacteriodes fragilis is an obligate anaerobe . It is identified by blackening of resentment esculin agar . Bile esculin agar contains esculin bile , protohemin , ferric ammonium citrate and gentamicin in a trypticase soy sauce agar base . The growth of the organism uses up bile and hydrolyses esculin1 . What is the significance of seeing spirochetes in Vincent s dye slidesSpirochetes when seen in Vincent s stained slides are indicative of the bacteria Borrelia vincentii . When the are large numbers of Gram-negative rods that appear spiral-like the test is positive for Borrelia vincentii the precipitating(prenominal) organism for Vincent s angina ( measuring rods Unit Evaluations and Standards Laboratory , 2007Section 6 .61 . What are the principles of tone of enunciate self-reliance you can interpret in a timber assurance reportThe information generated from the laboratory in a step assurance report should be accurate by following guidelines in the procedure manual , concerning tolerance units , acceptability of specimen preparation of reagents and the correct calculations . reliability is another principle that is pendant on the use of well qualified force for the various complexities of work , writing of reports on prescribed forms and postcode of nonindulgent actions as well as reporting anomalies to supervisorsReproducibility is also a principle of fibre assurance . It ensures that high tincture results can be obtained more than one time hence making them highly credible (WHO , 20061 . What are internal quality control , external quality appraisal random magisterial geological faults mean , SD , CV , imprecision , inaccuracy and biasWhere X mean n number of determine ValueCV is the coefficient of variation and provides measure of variability It can be used in equation of methods which generate different units It is obtained by dividing standards deviation by the mean and presenting as a piece by multiplying by 100Imprecision refers to errors resulting from compromised standards for example using a swampy lens or unwell prepared reagents . It results in arrogant errors . Inaccuracy is those errors that result form dick that have been facility disadvantageously or that are awry(p) resulting in harm readings (Medialab inc , 2008Bias refers to a situation where the testing process is altered often by a systematic error causing the results to a conjuring trick of the true facts (Medialab inc , 20081 . Explain the use of Westgard Rules and bill Jennings plotsWestgard rules are important for definition or performance limits for an assay . They are therefore useful in detection of random and systematic errors . They rules are in two sets , terce warning rules and three mandatory rules . The warning rules when violated initiate the review of calibration of equipment , test procedures and reagents use . Mandatory results are important in decision making whether to reject serum samples of a patient in an assy . Levy Jennings plots display in written means the assay values of replicate control against time . When a control point is found outside the /-2SD line , the patients results cannot be reported because they are considered `out of control . When they fall within the cover lines they are considered `in control and can thus be reported (Medialab Inc , 20081 . What are the role and requirements of accreditation by CPA (UK ) ltdCPA (UK ) Ltd is the main accreditation body for medical laboratories in the UK . It has the role of ensuring that laboratories meet the required standards to ensure high quality work and promotion of quality health services the patients . The accreditation requirements of CPA are classified into set standards the organization and quality management system , the personnel , set forth and environment , equipment , materials and information systems , the process of examination , pre-examination process and post examination processes . The laboratory is required to provide curtilage of quality in the management system , of qualified personnel and commensurate staffing records that show the quality management system is effective and examine of properly functioning equipment and a suitable working environment (CPA (UK ) Ltd , 20062 . Describe the different roles of the master and regulatory organization , for biomedical students such as IBMS , NPC and what is the relevance to nonrecreational practiceProfessional and regulatory organizations for biomedical students are necessary and have the responsibility of maintaining high standards of professional training and professional conduct in biomedicine . IBMS awards certificates of competence to biomedicine students and enables the students to account with HPC . IBMS also appoints verifiers , assess academician qualifications and approves laboratories for training of students before they go through . These organizations are relevant to the professional practice because they ensure professionalism is compound and maintained by allowing only competent biomedicine students join the organizationsReferencesAllen AV , Ridley DS , Further observations on the formol-ether concentration technique for faecal parasites . 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